Identification of n-terminal myristoyltransferase enzymes in bovine retina

Determination of kinetic parameters of type I and type II enzymes using multiple substrates
  • 1.26 MB
  • 2341 Downloads
  • English
by
[s.n.]
Acyltransferases, Cattle, Enzyme Inhibitors, Kinetics, Models, Biological, Retina, Substrate Specificity, enzymology, metab
The Physical Object
FormatUnknown Binding
ID Numbers
Open LibraryOL10917395M
ISBN 100599979917
ISBN 139780599979918
OCLC/WorldCa45687939

Protein N-myristoylation is a lipidic modification which refers to the covalent attachment of myristate, a carbon saturated fatty acid, to the N-terminal glycine residue of a number of mammalian, viral, and fungal proteins.

In this paper, we have cloned the gene coding for myristoyl-CoA:protein N-myristoyltransferase (NMT) from Bos tarus by: 1. Protein N-myristoylation is a lipidic modification which refers to the covalent attachment of myristate, a carbon saturated fatty acid, to the N-terminal glycine residue of a number of mammalian, viral, and fungal proteins.

In this paper, we have cloned the gene coding for myristoyl-CoA:protein N-myristoyltransferase (NMT) from Bos tarus brain. The open reading frame codes for a Cited by: 1. Volumenumber 1 FEBS LETTERS July N-TERMINAL SEQUENCE HOMOLOGY AMONG RETINOID-BINDING PROTEINS FROM BOVINE RETINA John W.

CRABB and John C. SAARI Department of Ophthalmology, University of Washington School of Medicine, Seattle, WAUSA Received 1 May by: Biochemical Characterization of Bovine Brain Myristoyl-CoA:Protein N-Myristoyltransferase Type 2 Article (PDF Available) in Journal of Biomedicine and Biotechnology () N-TERMINAL SEQUENCE HOMOLOGY AMONG RETINOID-BINDING PROTEINS FROM BOVINE RETINA John W.

CRABB and John C. SAARI Department of Ophthalmology, University of Washington School of Medicine, Seattle, WAUSA Received 1 May 1. Introduction Bovine retina contains at least 4 proteins which. Identification and characterization of multiple forms of bovine brain N-myristoyltransferase.

catalyzes the co-translational addition of myristic acid to the N-terminal glycine of many cellular, viral, and fungal proteins which are essential to normal cell functioning and/or are potential therapeutic targets.

We have found that bovine brain Cited by: Western blotting techniques were used with antibodies raised against human alpha, mu, and pi classes of GST enzymes. By comparing amino acid sequences of the N-terminal region of bovine ocular GSTs with those of human GST isozymes, a study was made of the structural relatedness of bovine ocular GST with human GST isozymes.

Description Identification of n-terminal myristoyltransferase enzymes in bovine retina FB2

The N-terminal sequence of bovine cystatin C was conclusively verified by tandem mass spectrometry. The C-terminal fragment ion series obtained with a sample in which free carboxyl groups had been esterified with methanol corresponds to the peptide sequence pyro-EGPRK ().This sequence was confirmed by the series of N-terminal fragments of the unmodified peptide (), thus identifying the N Cited by: 6.

N-Myristoyl-CoA: protein N-myristoyltransferase (NMT) is the enzyme that catalyses the covalent transfer of myristic acid from myristoyl-CoA to the N-terminal glycine residue of a protein substrate.

Identification of Codon Changes in the N-terminal Domain of YopH That Interfere with Binding to Cas in the Two-hybrid System.

The DNA sequence coding for the first residues of YopH was subjected to random mutagenesis using a PCR under suboptimal conditions ().The PCR was performed using pBD-YopH1–M45 as template and the oligonucleotides 5′PBD2H (5′.

title = "Targeting the role of N-terminal methionine processing enzymes in Mycobacterium tuberculosis", abstract = "The discovery of anti-tuberculosis agents that target new pathways is crucial for effective short-term TB therapy that will limit the development of by: 8.

Abstract – N-myristoyltransferase (NMT) attaches a 14 carbon fatty acid, myristic acid, to the N-terminal glycine residue of proteins.

NMT exists in two isoforms NMT1 and NMT2. Myristoylated proteins play critical roles in protein–protein interactions, cell signaling and oncogenesis. Although elevated expression.

Jeffrey I. Gordon obtained his A.B. from Oberlin College in and his M.D. from the University of Chicago in He then became an intern and junior assistant resident at Barnes Hospital in St. Louis before spending 3 years as a research associate in the Laboratory of Biochemistry at the National Cancer Institute (National Institutes of.

Compared to other species that possess a single functional myristoyl-CoA: protein N-myristoyltransferase gene copy, human, mouse and cow possess 2 NMT genes, and more than 2 protein isoforms. In mammals, the contribution of each gene transcript to multiple protein isoform expression and enzyme activity remains by: Myristate, a rare carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions.

N-myristoyltransferase (NMT\; EC ) catalyzes the transfer of myristate from CoA to proteins. Proteomics15, – DOI /pmic REVIEW N-terminal modifications of cellular proteins: The enzymes involved, their substrate specificities and biological effects Sylvia Varland1 ∗, Camilla Osberg1,2 and Thomas Arnesen1,2 1 Department of Molecular Biology, University of Bergen, Bergen, Norway 2 Department of Surgery, Haukeland University Hospital, Bergen, Norway.

N-Terminal Methionine Removal and Methionine Metabolism in Saccharomyces cerevisiae Benjamin Dummitt, William S. Micka, and Yie-Hwa Chang* Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, S.

N-terminal acetylation removes the charge from the amino terminus of a peptide. In general, acetyl modification is recommended if a peptide is meant to imitate its natural structure in a protein.

In addition, this modification stabilizes the resulting peptide towards enzymatic degradation resulting from. Recombinant bovine somatotropin (rbST), also called growth hormone, is a protein hormone used in dairy farming to enhance milk production. A method has been developed for the detection of rbST in milk by ESI(+)-LC-MS/MS.

This method allowed a detection limit of 20 pg of tryptic N-terminal peptide rbST in standard solution injected on.

Arylalkylamine N-acetyltransferase (AANAT) catalyzes the penultimate step in the biosynthesis of melatonin and other N-acetylarylalkylamides from the corresponding arylalkylamine and acetyl-CoA. The N-acetylation of arylalkylamines is a critical step in Drosophila melanogaster for the inactivation of the bioactive amines and the sclerotization of the cuticle.

title = "Protein N-terminal processing: Substrate specificity of escherichia coli and human methionine aminopeptidases", abstract = "Methionine aminopeptidase (MetAP) catalyzes the hydrolytic cleavage of the N-terminal methionine from newly synthesized by: Recombinant Human NF-L Protein.

Backed by our % Guarantee. Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains.

REVIEW CELL DEATH PATHWAYS Programmed necrosis in inflammation:Toward identification of the effector molecules David Wallach,1* Tae-Bong Kang,2 Christopher P.

Dillon,3 Douglas R. Green3* Until recently, programmed cell death was conceived of as a single set of molecular. 2 Preparing Samples for Protein Sequencing Protein Sequencing Overview Protein sequencing provides information about the amino acids that make up a protein.

During the sequencing process, amino acids are sequentially removed from the N-terminal end of the protein strand, and identified in the order they occur in the Size: KB.

Details Identification of n-terminal myristoyltransferase enzymes in bovine retina EPUB

A new cysteine protease named Nivulian-II has been purified from the latex of Euphorbia nivulia Buch.-Ham. The apparent molecular mass of Nivulian-II is Da (MALDI TOF/MS).

Peptide mass fingerprint analysis revealed peptide matches to Maturase K (Q52ZV1_9MAGN) of Banksia quercifolia. The N-terminal sequence (DFPPNTCCCICC) showed partial homology with those of other cysteine Cited by: 7. The Arg/N-end rule pathway targets for degradation proteins that bear specific unacetylated N-terminal residues while the Ac/N-end rule pathway targets proteins through their N^α-terminally acetylated (Nt-acetylated) residues.

Here, we show that Ubr1, the ubiquitin ligase of the Arg/N-end rule pathway, recognizes unacetylated N-terminal methionine if it is followed by a hydrophobic by: Rabies virus glycoprotein (RVG) is a trimeric ligand for the N-terminal cysteine-rich domain of the mammalian p75 neurotrophin receptor.

Christelle Langevin, Hanna Jaaro, Stéphane Bressanelli, Mike Fainzilber, Christine Tuffereau. Research output: Contribution to journal › by: Peptide Mass Fingerprinting and N-Terminal Amino Acid Sequencing of Glycosylated Cysteine Protease of Euphorbia nivulia Buch.-Ham. ar 1,2 n 2 Department of Biotechnology, Faculty of Science, Post Graduate College of Science, Technology and Research, North Maharashtra University, Jalgaon, Maharashtra, India.

Parvoviruses encode a large nonstructural protein 1 (NS1) that is essential for replication of the viral single-stranded DNA genome and DNA packaging and may play versatile roles in virus-host interactions.

Here, we report the structure of the human bocavirus NS1 N-terminal domain, the first for any autonomous parvovirus. efficient and easy-to-handle tool for the N-terminal modification of proteins based on an intermolecular transthioesterification reaction.

For N-terminal labeling, a protein containing an N-terminal cysteine undergoes a native chemical ligation with thioester probes.

The kit contains Coumarin-Thioester as fluorescent label. Background. @article{osti_, title = {Bovine leukemia virus nucleocapsid protein is an efficient nucleic acid chaperone}, author = {Qualley, Dominic F., E-mail: [email protected] and Sokolove, Victoria L.

and Ross, James L.}, abstractNote = {Nucleocapsid proteins (NCs) direct the rearrangement of nucleic acids to form the most thermodynamically stable structure, and facilitate many steps.

Download Identification of n-terminal myristoyltransferase enzymes in bovine retina EPUB

The N-terminal pyroglutamate of recombinant onconase is critical for its structural integrity, catalytic activity, and cyto-toxicity. On the basis of N-terminal sequence information in the protein database, 85%–90% of recombinant proteins should be produced in .Incorporation of unnatural amino acids into recombinant proteins represents a powerful tool for protein engineering and protein therapeutic development.

While the processing of the N-terminal methionine (Met) residues in proteins is well studied, the processing of unnatural amino acids used for replacing the N-terminal Met remains largely unknown.